Summary: Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei. Here. we describe a step-by-step protocol optimized for adult mouse skeletal muscles. This protocol provides two main advantages compared to the widely used single-cell protocol. https://www.bekindtopets.com/hot-mega-Jeffers-Economy-600D-Royal-Blue-Teal-Plaid-Horse-Blanket-super-sale/